20210223

Contents
  1. 1. Working directories
  2. 2. BLAST display
  3. 3. Primer BLAST
  4. 4. HEV
  5. 5. R
    1. 5.1. ggtree installation

Working directories

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i=/media/ht/ht_5T/Database/Virus/Herpesvirus/Herpesviridae; pcmanfm -n ${i}; cd ${i}
#
i=/media/ht/ht_5T/Database/Virus/Herpesvirus
pcmanfm -n ${i}; cd ${i}
#
i=/media/ht/ht_5T/Database/Virus/Herpesvirus/Herpesvirales_RefSeq.fas
FasCount ${i} # Output, 61
#
i=/media/ht/ht_5T/Database/Virus/Herpesvirus/Herpesviridae/Herpesviridae_RefSeq_20150320.fas
FasCount ${i} # Output, 73
#
i=/media/ht/ht_5T/Work/Projects/Herpesvirus_Project/EBV_CMV_Detect/20210219
pcmanfm -n ${i}; cd ${i}
#
i=/media/ht/ht_5T/Work/Projects/Herpesvirus_Project/EBV_CMV_Detect/20210219
pcmanfm -n ${i}; cd ${i}
Query=/media/ht/ht_5T/Work/Projects/Herpesvirus_Project/EBV_CMV_Detect/20210219/Quantitative_multi-target_RNA_profiling_in_EBV_infected_tumor_cells.fas
Subject=/media/ht/ht_5T/Database/Virus/Herpesvirus/Herpesviridae/Herpesviridae_RefSeq_20150320.fas
BLASTn ${Query} ${Subject}

BLAST display

Primer BLAST

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##################################################################
# BLASTn XXX YYY
##################################################################
function BLASTn {
  Query=$1; Subject=$2
  QueryBase=`basename ${Query}`; SubjectBase=`basename ${Subject}`; Tag=`date +%Y%m%d_%H%M`
  # blastn -query ${Query} -subject ${Subject} -outfmt 11 > blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.asn -max_hsps 10000 -num_alignments 10000
  # blast_formatter -archive blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.asn -outfmt 6 -out blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.csv; blast_formatter -archive blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.asn -line_length 100 -outfmt 0 -out blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.txt
  blastn -query ${Query} -subject ${Subject} -outfmt 6 > blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.csv -max_target_seqs 10000 -word_size 7
  blastn -query ${Query} -subject ${Subject} -outfmt 0 -line_length 100 > blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.txt -max_hsps 10000 -num_alignments 10000 -word_size 7
  blastn -query ${Query} -subject ${Subject} -outfmt 17 > blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.sam -max_target_seqs 10000 -word_size 7
  Query=$2; Subject=$1
  QueryBase=`basename ${Query}`; SubjectBase=`basename ${Subject}`
  # blastn -query ${Query} -subject ${Subject} -outfmt 11 > blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.asn -max_hsps 10000 -num_alignments 10000
  # blast_formatter -archive blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.asn -outfmt 6 -out blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.csv; blast_formatter -archive blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.asn -line_length 100 -outfmt 0 -out blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.txt
  blastn -query ${Query} -subject ${Subject} -outfmt 6 > blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.csv -max_target_seqs 10000 -word_size 7
  blastn -query ${Query} -subject ${Subject} -outfmt 0 -line_length 100 > blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.txt -max_hsps 10000 -num_alignments 10000 -word_size 7
  blastn -query ${Query} -subject ${Subject} -outfmt 17 > blastn_${QueryBase%.*}_vs_${SubjectBase%.*}_${Tag}.sam -max_target_seqs 10000 -word_size 7
  echo -e ${Green}'============================================'${NoColor}
  echo -e ${Green}'=============== BLASTn was Done ! ==============='
  echo -e ${Green}'============================================'${NoColor}
}

HEV

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Subject=/media/ht/ht_5T/Work/Funding/2017_重大专项/Management/Administration/09_02_63_HEV/Seq/HEV_25.fasta
Query=/media/ht/ht_5T/Work/Funding/2017_重大专项/Management/Administration/09_02_63_HEV/Seq/20210223/HEV_20210131_Filtrated.fas # Extracted w/ location and sampling date
#
BLASTn ${Query} ${Subject}
#
i=/media/ht/ht_5T/Work/Funding/2017_重大专项/Management/Administration/09_02_63_HEV/Seq/HEV_25.fasta
cat ${i} | ag '>'
cat ${i} | ag '>' | wc -l

R

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install.packages(c("boot", "broom", "cachem", "cli", "cluster", "crayon", "crul", "data.table", "dbplyr", "deldir", "dotCall64", "dplyr", "gert", "ks", "lifecycle", "MASS", "mgcv", "mime", "nlme", "pillar", "pkgKitten", "promises", "qpcR", "rappdirs", "RcppArmadillo", "rgdal", "rgl", "rJava", "rmarkdown", "segmented", "sf", "SparseM", "spatstat.data", "spdep", "testthat", "usethis", "waldo", "xfun", "XLConnect"))

ggtree installation

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if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("ggtree")

Expand all items >>

20210220

Contents
  1. 1. Working directories
  2. 2. 超详细Hexo+Github博客搭建小白教程 - 知乎
  3. 3. https://www.tandfonline.com/doi/pdf/10.4161/rna.27488
  4. 4. EBV Genomes
    1. 4.1. Nucleotide database

Working directories

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i=/media/ht/ht06/DB/SRA/Results/Essential/Extracted
pcmanfm -n ${i}; cd ${i}
#
i=/home/ht/Downloads/DB/SRA/Extracted
pcmanfm -n ${i}; cd ${i}
#
i=/media/ht/ht_5T_5/HLA-G/SRA/E-MTAB-1733/Results/Essential
pcmanfm -n ${i}; cd ${i}
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i=/media/ht/ht_5T/Work/Funding/2021_十四五/第四批12个社会发展领域2021年度项目申报指南征求意见/十四五指南_Sum.pdf; setsid okular ${i}
#
i=/media/ht/ht_5T/Work/Funding/2021_十四五/第四批12个社会发展领域2021年度项目申报指南征求意见/十四五指南_Sum.pdf
setsid okular ${i}

超详细Hexo+Github博客搭建小白教程 - 知乎

以上教程非常有用。

https://www.tandfonline.com/doi/pdf/10.4161/rna.27488

EBV Genomes

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i=/media/ht/ht_5T/Work/Projects/Herpesvirus_Project/EBV_CMV_Detect/20210219/EBV_B95-8_V01555.2.gb
subl ${i}
#
i=/media/ht/ht_5T/Work/Projects/Herpesvirus_Project/EBV_CMV_Detect/20210219/EBV_NC_009334.gb
subl ${i}
#
i=/media/ht/ht_5T/Work/Projects/Herpesvirus_Project/EBV_CMV_Detect/20210219/EBV_All.acc
subl ${i}
#

Nucleotide database

Keyword
Human herpesvirus 4

“Human gammaherpesvirus 4”[Organism] OR Human herpesvirus 4[All Fields]

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i=/media/ht/ht_5T/Database/Virus/Herpesvirus/EBV
pcmanfm -n ${i}; cd ${i}
HHV4.summary
HHV4.gb
HHV4.fasta

Herpesviridae

“Herpesviridae”[Organism] OR Herpesviridae[All Fields]

Expand all items >>

20210219

Contents
  1. 1. Working directories
  2. 2. EBV detection primer sets
    1. 2.1. My collection

Working directories

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i=/media/ht/ht_5T/Work/Projects/Phage
pcmanfm -n ${i}; cd ${i}
#
i='/media/ht/ht_5T/Work/Projects/NFE/Publication/Draft/Rev_2'
pcmanfm -n ${i}; cd ${i}
#
i=/media/ht/ht_5T/Work/Projects/NFE/Flu
pcmanfm -n ${i}; cd ${i}
#
i=/media/ht/ht_5T/Bioinformatics/软件使用说明_电影版/Not_Classified/QQ链接网页内容保存为Word文档方法_Android系统.mp4
ls ${i} # QQ链接网页内容保存为Word文档方法_Android系统.mp4, Storage

EBV detection primer sets

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i=/media/ht/ht_5T/Work/Projects/NFE/B95-8/Ref; pcmanfm -n ${i}; cd ${i}
  • 101_Chaetocin_reactivates_the_lytic_replication_of_EBV_from_latency_via_reactive_oxygen_species.pdf
  • 184_Pharmacologic_Activation_of_Lytic_EBV_Gene_Expression_without_Virion_Production.pdf
  • 186_Inhibition_of_class_I_histone_deacetylases_by_romidepsin_potently_induces_EBV_lytic_cycle_ganciclovir.pdf
  • 203_The_mTOR_Inhibitor_Manassantin_B_Reveals-A_Crucial_Role_of_mTORC2_Signaling_in_EBV_Reactivation.pdf
  • 220_Emodin_Inhibits_EBV_Reactivation_and_Represses_NPC_Tumorigenesis.pdf
  • 222_Rapamycin_enhances_lytic_replication_of_EBV_in_gastric_carcinoma_cells_by_increasing_the_transcriptional_of_lytic_promoters.pdf
  • 225_Genipin_as_a_novel_chemical_activator_of_EBV_lytic_cycle.pdf
  • 226_Cordycepin_is_a_novel_chemical_suppressor_of_EBV.pdf
  • 227_Quercetin-induced_apoptosis_prevents_EBV_infection.pdf

My collection

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i=/media/ht/ht_5T/Work/Projects/Herpesvirus_Project/EBV_CMV_Detect/20210219; pcmanfm -n ${i}; cd ${i}
#
i=/media/ht/ht_5T/Work/Projects/Herpesvirus_Project/EBV_CMV_Detect/20210219/EBV_Detect_Ref_Sum.pdf; setsed okular ${i}
  • /media/ht/ht_5T/Work/Projects/Herpesvirus_Project/Herpesvirus_Latent_Gene/Ref/EBV_splicing/Restricted_Expression_of_EBV_Latent_Genes_in_Murine_B_Cells.pdf
  • /media/ht/ht_5T/Work/Projects/NFE/B95-8/Ref/EBV_DNA_load_RT-PCR/184_Pharmacologic_Activation_of_Lytic_EBV_Gene_Expression_without_Virion_Production.pdf

Expand all items >>

20210217

Contents
  1. 1. Working directories
  2. 2. Bacteriophage RnD
    1. 2.1. 禁止抗生素相关文件
    2. 2.2. Patent collection from Google Patents
    3. 2.3. Intralytix
    4. 2.4. ContraFect
    5. 2.5. 菲吉乐科
    6. 2.6. Ref

Working directories

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i=ftp://ht@192.168.1.8:2121/BaiduNetdisk/resources
pcmanfm -n ${i}; cd ${i}
#
i=ftp://ht@192.168.1.7:2121/Download
pcmanfm -n ${i}; cd ${i}

Bacteriophage RnD

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i=/media/ht/ht_5T/Work/Projects/Phage
pcmanfm -n ${i}; cd ${i}

Bacteriophage presentation

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i=/media/ht/ht_5T/Work/Projects/Phage
pcmanfm -n ${i}; cd ${i} # Bacteriophage RnD Powerpoint updated
  1. Check the number of institutes, Phage_Directory.docx
  2. Check the number of patents
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i='/media/ht/ht_5T/Work/Projects/Phage/Summary'; cd ${i}
#
i='/media/ht/ht_5T/Work/Projects/Phage/Summary/Phage_Directory.docx'; setsid wps ${i} # Bacteriophage research institutes, 79 totally
#
i='/media/ht/ht_5T/Work/Projects/Phage/Summary/Intralytix.docx'; setsid wps ${i}
#
i='/media/ht/ht_5T/Work/Projects/Phage/Patents'; cd ${i}
#
i='/media/ht/ht_5T/Work/Projects/Phage/Patents/gp-search-20210217-003642.xlsx'; setsid et ${i}
#

Bacteriophage

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i=/media/ht/ht_5T/Work/Projects/Phage/README.md
subl ${i} # patents summarized
#
i=/media/ht/ht_5T/Work/Projects/Phage/Summary
pcmanfm -n ${i}; cd ${i}

禁止抗生素相关文件

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i='/media/ht/ht_5T/Work/Projects/Phage/Materials'
cd ${i}
pcmanfm -n ${i}
#
i='/media/ht/ht_5T/Work/Projects/Phage/Materials/Domestic_Info.docx'; setsid wps ${i} # updated 20210322
# 
i='/media/ht/ht_5T/Work/Projects/Phage/Materials/Domestic_Info.pdf'; setsid okular ${i} # updated 20210322

Patent collection from Google Patents

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i=/media/ht/ht_5T/Work/Projects/Phage/Patents
pcmanfm -n ${i}; cd ${i}

Intralytix

Intralytix, Inc. is a biotechnology company focused on the discovery, production and marketing of bacteriophage-based products to control bacterial pathogens in environmental, food processing, and medical settings.

Bacteriophages (bacteria-eater: from the Greek phago meaning “to eat” or “to develop at the expense of”) are the most abundant microorganisms on earth. They are the natural enemies of bacteria. Lytic bacteriophages can very effectively kill their targeted specific bacteria without affecting anything else, ensuring an unprecedented level of safety for an antimicrobial.

ContraFect

ContraFect

菲吉乐科

Ref

Expand all items >>

20210216

Contents
  1. 1. Working directories
  2. 2. Availability of subsurface water-ice resources in the northern mid-latitudes of Mars | Nature Astronomy
  3. 3. Mobile FTP
  4. 4. FTP/Mobile phones
    1. 4.0.1. DCIM/Camera Directory
    2. 4.0.2. Download Directory
    3. 4.0.3. 百度网盘
    4. 4.0.4. 微信
    5. 4.0.5. QQ
    6. 4.0.6. COVID-19
    7. 4.0.7. Study Directory
    8. 4.0.8. Markor Directory
  • 5. COVID-19 data
  • 6. Glossary of COVID-19
    1. 6.1. GAVI
    2. 6.2. COVAX

    • Working directories

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    i=/media/ht/ht_5T/Work/Projects/NFE/Summary/NFE2_Sum_Large.pdf
    setsid okular ${i}
    #
    i=/media/ht/ht_5T/Work/Projects/NFE/Summary/NFE2_Sum_Large.pptx
    setsid wpp ${i}

    Availability of subsurface water-ice resources in the northern mid-latitudes of Mars | Nature Astronomy

    SWIM

    Mobile FTP

    FTP/Mobile phones

    DCIM/Camera Directory

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    Site=ftp://ht@192.168.1.${i}:2121/DCIM; pcmanfm -n ${Site} # DCIM/Camera Directory
    #
    Site='/run/user/1000/gvfs/ftp:host=192.168.1.'${i}',port=2121,user=ht/'; Site=${Site}"DCIM"; pcmanfm -n ${Site}; cd ${Site}

    Download Directory

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    Site=ftp://ht@192.168.1.${i}:2121/Download; pcmanfm -n ${Site} # Download directory
    #
    i='/run/user/1000/gvfs/ftp:host=192.168.1.8,port=2121,user=ht/'
    #
    Site='/run/user/1000/gvfs/ftp:host=192.168.1.'${i}',port=2121,user=ht/'; Site=${Site}"Download"; pcmanfm -n ${Site}; cd ${Site}

    百度网盘

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    Site=ftp://ht@192.168.1.${i}:2121/BaiduNetdisk; pcmanfm -n ${Site} # 百度网盘
    #
    Site='/run/user/1000/gvfs/ftp:host=192.168.1.'${i}',port=2121,user=ht/'; Site=${Site}"BaiduNetdisk"; pcmanfm -n ${Site}; cd ${Site}

    微信

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    Site=ftp://ht@192.168.1.${i}:2121/Android/data/com.tencent.mm/MicroMsg/Download; pcmanfm -n ${Site} # WeChat/Weixin
    #
    Site='/run/user/1000/gvfs/ftp:host=192.168.1.'${i}',port=2121,user=ht/'; Site=${Site}"Android/data/com.tencent.mm/MicroMsg/Download"; pcmanfm -n ${Site}; cd ${Site}

    QQ

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    Site=ftp://ht@192.168.1.${i}:2121/Android/data/com.tencent.mobileqq/Tencent/QQfile_recv; pcmanfm -n ${Site} ## Mobile QQ
    #
    Site='/run/user/1000/gvfs/ftp:host=192.168.1.'${i}',port=2121,user=ht/'; Site=${Site}"Android/data/com.tencent.mobileqq/Tencent/QQfile_recv"; pcmanfm -n ${Site}; cd ${Site}

    COVID-19

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    Site=ftp://ht@192.168.1.${i}:2121/Documents/Study/COVID-19; pcmanfm -n ${Site} # COVID-19
    #
    Site='/run/user/1000/gvfs/ftp:host=192.168.1.'${i}',port=2121,user=ht/'; Site=${Site}"Documents/Study/COVID-19"; pcmanfm -n ${Site}; cd ${Site}

    Study Directory

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    Site=ftp://ht@192.168.1.${i}:2121/Documents/Study/; pcmanfm -n ${Site} # Study
    #
    Site='/run/user/1000/gvfs/ftp:host=192.168.1.'${i}',port=2121,user=ht/'; Site=${Site}"Documents/Study/"; pcmanfm -n ${Site}; cd ${Site}

    Markor Directory

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    Site=ftp://ht@192.168.1.${i}:2121/Documents/markor/; pcmanfm -n ${Site} # Markor
    #
    Site='/run/user/1000/gvfs/ftp:host=192.168.1.'${i}',port=2121,user=ht/'; Site=${Site}"Documents/markor/"; pcmanfm -n ${Site}; cd ${Site}

    COVID-19 data

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    mkdir `Date`;cd `Date`; pcmanfm -n 
    #
    i='https://github.com/CSSEGISandData/COVID-19/trunk/csse_covid_19_data/csse_covid_19_time_series'; time svn checkout ${i}
    #
    cd ./csse_covid_19_time_series; 7z a ../../COVID-19_`date +%Y%m%d`.7z *.*; cd ../; find ./ -type f -name "*.csv" | xargs -I % mv % ./; rm -r csse_covid_19_time_series/; rm Errata.csv; Corona
    #
    i='https://github.com/CSSEGISandData/COVID-19/trunk/csse_covid_19_data/csse_covid_19_time_series'; svn checkout ${i}
    #
    find . -type f -name "*.csv" -exec mv {} ./ \;
    #
    svn checkout https://github.com/CSSEGISandData/COVID-19/trunk/csse_covid_19_data/csse_covid_19_time_series
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    for i in 'https://raw.githubusercontent.com/CSSEGISandData/COVID-19/master/csse_covid_19_data/csse_covid_19_time_series/time_series_covid19_confirmed_global.csv https://raw.githubusercontent.com/CSSEGISandData/COVID-19/master/csse_covid_19_data/csse_covid_19_time_series/time_series_covid19_deaths_global.csv https://raw.githubusercontent.com/CSSEGISandData/COVID-19/master/csse_covid_19_data/csse_covid_19_time_series/time_series_covid19_recovered_global.csv https://raw.githubusercontent.com/CSSEGISandData/COVID-19/master/csse_covid_19_data/csse_covid_19_time_series/time_series_covid19_confirmed_US.csv https://raw.githubusercontent.com/CSSEGISandData/COVID-19/master/csse_covid_19_data/csse_covid_19_time_series/time_series_covid19_deaths_US.csv'; do wget ${i}; done
    #
    i='https://raw.githubusercontent.com/CSSEGISandData/COVID-19/master/csse_covid_19_data/csse_covid_19_time_series/time_series_covid19_confirmed_global.csv https://raw.githubusercontent.com/CSSEGISandData/COVID-19/master/csse_covid_19_data/csse_covid_19_time_series/time_series_covid19_deaths_global.csv https://raw.githubusercontent.com/CSSEGISandData/COVID-19/master/csse_covid_19_data/csse_covid_19_time_series/time_series_covid19_recovered_global.csv https://raw.githubusercontent.com/CSSEGISandData/COVID-19/master/csse_covid_19_data/csse_covid_19_time_series/time_series_covid19_confirmed_US.csv https://raw.githubusercontent.com/CSSEGISandData/COVID-19/master/csse_covid_19_data/csse_covid_19_time_series/time_series_covid19_deaths_US.csv'; setsid chromium-browser --new-window ${i}
    #
    i=
    Site=ftp://ht@192.168.1.${i}:2121/Documents/Study/COVID-19; pcmanfm -n ${Site} # COVID-19

    Glossary of COVID-19

    GAVI

    The GAVI Alliance (formerly the Global Alliance for Vaccines and Immunisation) is a global health partnership of public and private sector organizations dedicated to “immunisation for all”. It provides a unique opportunity for a wide-range of partners to build consensus around policies, strategies, and priorities and to recommend responsibility of implementation to the partner with the most experience and insight in the area. GAVI has developed innovative approaches to international health and development.

    COVAX

    COVID-19 Vaccines Global Access, abbreviated as COVAX, is a global initiative aimed at equitable access to COVID-19 vaccines led by the Global Alliance for Vaccines and Immunization, the World Health Organization, the Coalition for Epidemic Preparedness Innovations, and others.

    COVAX is one of three pillars of the Access to COVID-19 Tools (ACT) Accelerator, which was launched in April by the World Health Organization (WHO), the European Commission and France in response to this pandemic. Bringing together governments, global health organisations, manufacturers, scientists, private sector, civil society and philanthropy, with the aim of providing innovative and equitable access to COVID-19 diagnostics, treatments and vaccines. The COVAX pillar is focussed on the latter. It is the only truly global solution to this pandemic because it is the only effort to ensure that people in all corners of the world will get access to COVID-19 vaccines once they are available, regardless of their wealth.

    Expand all items >>

    20210214

    Contents
    1. 1. Working directories
      1. 1.1. Kaiju: Fast and sensitive taxonomic classification for metagenomics
      2. 1.2. Windows 10 VB download
    2. 2. NSFC Application
    3. 3. Accessable directory in a mobile phone with command-line
    4. 4. Vaccine in US
      1. 4.1. App, v-safe
      2. 4.2. Immuno-suppresive
      3. 4.3. Pfizer
      4. 4.4. Moderna
      5. 4.5. Sputnik V

    Working directories

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    i=/media/ht/ht_5T/Bioinformatics/Bioinfo-Softs/NCBI/Datasets
    pcmanfm -n ${i}; cd ${i} # NCBI Datasets command-line # See NCBI_Learning.html
    #
    # Remote BWA with Vision
    # E-MTAB-173, Essential
    #
    i=/media/ht/ht_5T/Work/Projects/Phage
    pcmanfm -n ${i}; cd ${i} # Phage RnD Powerpoint updated
    #
    i=/media/ht/ht_5T/Work/Projects/Phage/Phage_RnD_Background_20210214.pptx
    setsid wpp ${i} # Phage RnD Powerpoint updated
    #
    i='/media/ht/ht_5T/Work/Projects/Phage/Phage_RnD_Background.pptx'; setsid wps ${i}
    #
    i=/media/ht/ht_5T_9/YouTubes/History/Wuhan/Vaccine/CDC_美国接种4百万Moderna新冠疫苗后的严重过敏反应数据分析.mp4
    setsid vlc ${i}
    #
    i=/media/ht/ht_5T_9/YouTubes/English/German/德文字母及其發音介紹-母音及Umlaut發音練習-Das_deutsche_Alphabet-全程中文說明.mp4
    setsid vlc ${i}

    Kaiju: Fast and sensitive taxonomic classification for metagenomics

    Publication downloaded from URL as below

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    i=/media/ht/ht_5T/Bioinformatics/Bioinfo-Softs/NGS_Softs/Kaiju
    pcmanfm -n ${i}; cd ${i} # Kaiju

    Windows 10 VB download

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    i=https://az792536.vo.msecnd.net/vms/VMBuild_20190311/VirtualBox/MSEdge/MSEdge.Win10.VirtualBox.zip
    axel -q -n 10 ${i}
    Windows 10 Download Sites

    NSFC Application

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    #
    i='/media/ht/ht_5T/Work/Funding/2021_国家自然科学基金'; pcmanfm -n ${i}; cd ${i} # NSFC
    • 信息科学部案例, P6
    • Code, 医学, H2103, H1904. 肝炎病毒与感染
    • Code, 生命科学, C06, 生物信息理论方法

    Accessable directory in a mobile phone with command-line

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    i=/run/user/1000/gvfs/ftp:host=192.168.1.8,port=2121,user=ht/Download/
    pcmanfm -n ${i}; cd ${i}

    Vaccine in US

    Vaccine efficacy

    App, v-safe

    v-safe

    Priority and coverage of vaccine

    1. 5%
    2. 30%
    3. 40%

    Immuno-suppresive

    tacrolimus, cyclosporine, mycophenolate, azathioprine, sirolimus

    • EpiPen 2-Pak, 过敏

    Other names: Epinephrine, adrenaline, adrenalin

    Adrenaline, also known as epinephrine, is a hormone and medication which is involved in regulating visceral functions (e.g., respiration).. Adrenaline is normally produced both by the adrenal glands and by a small number of neurons in the medulla oblongata.

    Generic Name: epinephrine injection (EP i NEF rin)
    Brand Name: Adrenalin, Auvi-Q, Epinephrinesnap-EMS, Epinephrinesnap-V, EpiPen 2-Pak, EpiPen JR 2-Pak, EPIsnap, Symjepi

    EpiPen 2-Pak is used to treat severe allergic reactions (anaphylaxis) to insect stings or bites, foods, drugs, and other allergens.

    Epinephrine auto-injectors may be kept on hand for self-injection by a person with a history of severe allergic reaction.

    Epinephrine is also used to treat exercise-induced anaphylaxis, or to treat low blood pressure that is caused by septic shock.

    EpiPen 2-Pak may also be used for purposes not listed in this medication guide.

    Pfizer

    • 44 K
    • Placebo, 162, 9 heavy
    • Vaccine, 8, 1 heavy
    • 5 shots / vial, 0.3 ml / shot. Dilution needed

    Moderna

    • 30 K
    • Placebo, 185, 30 heavy
    • Vaccine, 11, 0 heavy
    • 10 shots / vial, 0.5 ml / shot

    The trial began on July 27, 2020, and enrolled 30,420 adult volunteers at clinical research sites across the United States. Volunteers were randomly assigned 1:1 to receive either two 100 microgram (mcg) doses of the investigational vaccine or two shots of saline placebo 28 days apart. The average age of volunteers is 51 years. Approximately 47% are female, 25% are 65 years or older and 17% are under the age of 65 with medical conditions placing them at higher risk for severe COVID-19. Approximately 79% of participants are white, 10% are Black or African American, 5% are Asian, 0.8% are American Indian or Alaska Native, 0.2% are Native Hawaiian or Other Pacific Islander, 2% are multiracial, and 21% (of any race) are Hispanic or Latino.

    From the start of the trial through Nov. 25, 2020, investigators recorded 196 cases of symptomatic COVID-19 occurring among participants at least 14 days after they received their second shot. One hundred and eighty-five cases (30 of which were classified as severe COVID-19) occurred in the placebo group and 11 cases (0 of which were classified as severe COVID-19) occurred in the group receiving mRNA-1273. The incidence of symptomatic COVID-19 was 94.1% lower in those participants who received mRNA-1273 as compared to those receiving placebo.

    Investigators observed 236 cases of symptomatic COVID-19 among participants at least 14 days after they received their first shot, with 225 cases in the placebo group and 11 cases in the group receiving mRNA-1273. The vaccine efficacy was 95.2% for this secondary analysis.

    • 0.2-0.3 % death rate (Mortality rate) of flu
    • 1.8 % death rate of COVID-19

    Sputnik V

    Sputnik V

    Expand all items >>

    20210213

    Contents
    1. 1. Working directories
    2. 2. p62 Draft
      1. 2.1. Figures
      2. 2.2. 全文布局
        1. 2.2.1. Introduction
        2. 2.2.2. Results
        3. 2.2.3. 讨论
    3. 3. Flatpak 新手指南 - 知乎

    Working directories

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    3
    4
    5
    6
    7
    8
    9
    i=/media/ht/ht_5T/Work/Projects/NFE/Publication/Draft/Rev_2
    pcmanfm -n ${i}; cd ${i}
    #
    i=/media/ht/ht_5T/Stored_Softs/Cross_Platform_Softs/Shotcut/Instruction
    pcmanfm -n ${i}; cd ${i} # Combine video instruction
    #
    i=/media/ht/ht_5T/Stored_Softs/Cross_Platform_Softs/Shotcut/Instruction/Instruction.mp4
    setsid vlc ${i} # Shotcut instruction
    ls /media/ht/ht_5T/Stored_Softs/Cross_Platform_Softs/Shotcut/Instruction/Instruction_Backup.7z # Video backup
    • 代码的魅力_COVID-19_Full.mkv
    • 代码的魅力_COVID-19.mkv
    1
    2
    3
    i=/media/ht/ht_5T/Bioinformatics/软件使用说明_电影版/Not_Classified
    i=/media/ht/ht_5T/Work/Projects/NFE/Publication/Draft/Rev_2
    pcmanfm -n ${i}; cd ${i}
    • Watch charging

    p62 Draft

    • [[20201229]]
    • [[20210126]]
    • [[20210127]]
    • [[20210130]]
    • [[20210201]]
    • [[20210202]]
    • [[20210203]]
    • [[20210204]]
    • [[20210205]]
    • [[20210206]]
    • [[20210212]]

    Figures

    • Fig 1, 293细胞，双转。完全没有问题
    • Fig 2, 293细胞，药物处理，E6/E7 transfection
      • 2B, Nrf2 (lanes 4 and 5)怎么比较，目前版本，信号没有差异。Nrf2没有差异的情况下，观察E6是否成立。
      • 2C, 没有问题
    • Fig 3, HeLa，药物处理
      • 3A, 没有问题
      • 3B, 应该分成两个panel。此外，差异是否有统计学意义。
    • Fig 4, 共聚焦
      • 4A, 对照，没有问题
      • 4B, Nrf2和E6分别在不同的细胞
      • 4C, 尤其Nrf2需要调整。获得什么结论？
    • Fig 5, IVTT, 完全没有问题

    全文布局

    Introduction

    • ROS
      • ROS重要性，与Oxident stress联系
      • Nrf2是ROS的核心机制之一。引出ARE
      • Nrf2/ROS与肿瘤进展的相关性
    • 宫颈癌
      • 属于一种重要的肿瘤
      • HPV
      • HPV与ROS的相关性
      • HPV的E6/E7与Nrf2的观察，没有发生过

    Results

    • 双转显示Nrf2的上调与E6/E7的下调同时出现
    • 药物(tBHQ)刺激上调内生Nrf2导致转染E6/E7下调
    • 药物(tBHQ)刺激上调内生Nrf2上调与HeLa细胞中的E6/E7上调同步
    • Nrf2与E6/E7分布于不同的细胞
    • IVTT显示Nrf2的上调与E6/E7的下调同时出现

    讨论

    • 实验技术
      • Nrf2的双带
      • E6检测的难点
    • Nrf2抑制E6/E7
    • Nrf2促进E6/E7
    • Nrf2间接影响E6/E7

    Flatpak 新手指南 - 知乎

    Expand all items >>

    20210212

    Contents
    1. 1. Working directories

    Working directories

    1
    2
    3
    4
    i=/media/ht/ht_5T/Work/Funding/2017_重大专项/Management/Administration/09_02_63_HEV/CNN
    pcmanfm -n ${i}; cd ${i} # Backup Yao Yuan's output
    # /media/ht/ht_5T/Work/Funding/2017_重大专项/Management/Administration/09_02_63_HEV/CNN/filter_both.csv
    # /media/ht/ht_5T/Work/Funding/2017_重大专项/Management/Administration/09_02_63_HEV/CNN/dna_distribution.svg

    Expand all items >>

    {% if theme.mermaid.enable %} {% endif %}

    tag:

      缺失模块。
      1、请确保node版本大于6.2
      2、在博客根目录（注意不是yilia根目录）执行以下命令：
      npm i hexo-generator-json-content --save

      3、在根目录_config.yml里添加配置： 

        jsonContent:
    meta: false
    pages: false
    posts:
      title: true
      date: true
      path: true
      text: false
      raw: false
      content: false
      slug: false
      updated: false
      comments: false
      link: false
      permalink: false
      excerpt: false
      categories: false
      tags: true

    Step by step.