20210317

Contents
  1. 1. Working directories
    1. 1.1. CEB format
      1. 1.1.1. CEB 文件摘要
      2. 1.1.2. CEB/Word conversion
    2. 1.2. Script in ht_5T_9 and ht_T7_1
  2. 2. Hexo tips
  3. 3. Virus proliferation cell free system
    1. 3.1. Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
      1. 3.1.1. Abstract
    2. 3.2. Cell-free RNA replication systems based on a human cell extracts-derived in vitro translation system with the encephalomyocarditisvirus RNA
      1. 3.2.1. Abstract
    3. 3.3. An improved cell-free system for picornavirus synthesis
      1. 3.3.0.1. Erratum in
    4. 3.3.1. Abstract
  4. 3.4. Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
    1. 3.4.1. Abstract
  5. 3.5. A Cell-Free Assembly System for Generating Infectious Human Papillomavirus 16 Capsids Implicates a Size Discrimination Mechanism for Preferential Viral Genome Packaging
    1. 3.5.1. ABSTRACT
  6. 3.6. Cell-free synthesis of encephalomyocarditis virus
    1. 3.6.0.1. Abstract

Working directories

CEB format

CEB 文件摘要

根据我们的记录，有 一 种与 CEB 文件扩展名相关的文件类型，最常见的被格式化为 Apabi eBook File。Apabi Reader 由 Founder Group 发布，是最通用的关联应用程序。 此外，有 一 种不同的软件程序可供您用于查看这些文件。 大多数 CEB 文件被视为 eBook Files。

CEB 文件可以在移动和桌面平台上找到，可以在 Windows中打开。 这些文件的普及性为“低”，这意味着它们并不常见。

如果您在打开 CEB 文件时遇到问题，或者只是想了解有关它们的软件程序和开发人员的更多信息，请参阅下面的完整信息。

CEB/Word conversion

Script in ht_5T_9 and ht_T7_1

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i='/media/ht/ht_5T_9/YouTubes/README.sh'; subl ${i}
i='/media/ht/ht_T7_1/README.sh'; subl ${i}
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# y2mate.com
#
space; space; rename 's/\_720p//; s/\_240p//; s/\_360p//; s/\_480p//; s/\_1080p//; s/\_v720P//'  y2*0p.* y2*0P.*
Remove_cnmark; Remove_cnmark
#
for i in y2m*.*; do mv ${i} ${i:11:200}; done
for i in y2m*.*; do mv ${i} ${i:11:200}; done; for i in *.*; do mv ${i} `echo ${i} | rev | cut -b 17-100 | rev`.${i#*.*}; done

Hexo tips

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i='https://segmentfault.com/a/1190000017986794 https://hexo.io/zh-cn/docs/github-pages.html https://www.jianshu.com/p/7daa1dc0e117'; setsid chromium-browser ${i}

Virus proliferation cell free system

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i='https://jvi.asm.org/content/90/17/7607/article-info https://jvi.asm.org/content/jvi/90/17/7607.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838232/ https://pubmed.ncbi.nlm.nih.gov/21952914/ https://pubmed.ncbi.nlm.nih.gov/25488519/ https://pubmed.ncbi.nlm.nih.gov/21622667/ https://pubmed.ncbi.nlm.nih.gov/17320977/ https://pubmed.ncbi.nlm.nih.gov/12743313/ https://link.springer.com/article/10.1007%2Fs10529-011-0744-z'; setsid chromium-browser ${i}

Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube

Tominari Kobayashi 1, Yusuke Nakamura, Satoshi Mikami, Mamiko Masutani, Kodai Machida, Hiroaki Imataka

Affiliations expand

Abstract

Virus particles are used in vaccination, drug delivery, and material sciences. Here we devised a system where the RNA virus encephalomyocarditis virus (EMCV) is synthesized from DNA templates in vitro. When a plasmid or a PCR product harboring the full-length cDNA of EMCV in the T7 promoter/terminator unit was incubated in a HeLa cell extract supplemented with T7 RNA polymerase, EMCV was produced within 4 h at an efficiency of over 10-fold compared with the system programmed with the EMCV RNA. The EMCV RNA transcribed by the virally encoded RNA-dependent RNA polymerase was predominantly incorporated into the EMCV particle even in the presence of a larger amount of the EMCV RNA transcribed by T7 RNA polymerase from the plasmid.

Cell-free RNA replication systems based on a human cell extracts-derived in vitro translation system with the encephalomyocarditisvirus RNA

Tominari Kobayashi 1, Kodai Machida, Satoshi Mikami, Mamiko Masutani, Hiroaki Imataka

Affiliations expand

Abstract

To study the relationship between translation and replication of encephalomyocarditisvirus (EMCV) RNA, we established a cell-free RNA replication system by employing a human cell extracts-based in vitro translation system. In this system, a cis-EMCV RNA replicon encoding the Renilla luciferase (R-luc) or GFP and the viral regulatory proteins efficiently replicated with simultaneous translation of the encoded protein. To examine how translation of the replicon RNA, but not the translated products, affected replication, a trans-EMCV RNA replicon encoding R-luc and the RNA replication elements was next constructed. The trans-replicon RNA replicated only in the presence of the regulatory proteins pre-expressed in trans. Incubation with cycloheximide, puromycin or a dominant-negative eukaryotic translation initiation factor 4A following expression of the regulatory proteins almost completely inhibited not only translation of the trans-replicon RNA but also replication of the RNA, suggesting that EMCV RNA translation promotes replication of the RNA. In conclusion, the cell-free RNA replication systems should become useful tools for the study of the viral RNA replication.

An improved cell-free system for picornavirus synthesis

Tominari Kobayashi 1, Satoshi Mikami, Shigeyuki Yokoyama, Hiroaki Imataka

Affiliations expand

Erratum in

  • J Virol Methods. 2012 Jan;179(1):e1

Abstract

Cell-free synthesis of an infectious virus is an ideal tool for elucidating the mechanism of viral replication and for screening anti-viral drugs. In the present study, the synthesis of Encephalomyocarditis virus (EMCV) from its RNA in HeLa and 293-F cell extracts was enhanced by employing a dialysis system in combination with a ribozyme technology. Although translation and processing of the EMCV polyprotein were not accelerated greatly by the dialysis system, de novo synthesis of viral RNA was enhanced considerably by dialysis, leading to a greater than eight-fold increased titer of synthesized EMCV compared with a conventional batch system. Furthermore, a synthetic EMCV RNA with a hammerhead ribozyme sequence at its 5’-end served as an efficient template for viral synthesis in the dialysis system. Therefore, this system provides opportunities for mutational analyses of EMCV in vitro.

Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube

Biotechnology Letters volume 34, pages67–73(2012)Cite this article

Abstract

Virus particles are used in vaccination, drug delivery, and material sciences. Here we devised a system where the RNA virus encephalomyocarditis virus (EMCV) is synthesized from DNA templates in vitro. When a plasmid or a PCR product harboring the full-length cDNA of EMCV in the T7 promoter/terminator unit was incubated in a HeLa cell extract supplemented with T7 RNA polymerase, EMCV was produced within 4 h at an efficiency of over 10-fold compared with the system programmed with the EMCV RNA. The EMCV RNA transcribed by the virally encoded RNA-dependent RNA polymerase was predominantly incorporated into the EMCV particle even in the presence of a larger amount of the EMCV RNA transcribed by T7 RNA polymerase from the plasmid.

A Cell-Free Assembly System for Generating Infectious Human Papillomavirus 16 Capsids Implicates a Size Discrimination Mechanism for Preferential Viral Genome Packaging

Carla Cerqueira, Yuk-Ying S. Pang, Patricia M. Day, Cynthia D. Thompson, Christopher B. Buck, Douglas R. Lowy, John T. Schiller

S. Perlman, Editor

DOI: 10.1128/JVI.02497-15

ABSTRACT

We have established a cell-free in vitro system to study human papillomavirus type 16 (HPV16) assembly, a poorly understood process. L1/L2 capsomers, obtained from the disassembly of virus-like particles (VLPs), were incubated with nuclear extracts to provide access to the range of cellular proteins that would be available during assembly within the host cell. Incorporation of a reporter plasmid “pseudogenome” was dependent on the presence of both nuclear extract and ATP. Unexpectedly, L1/L2 VLPs that were not disassembled prior to incubation with a reassembly mixture containing nuclear extract also encapsidated a reporter plasmid. As with HPV pseudoviruses (PsV) generated intracellularly, infection by cell-free particles assembled in vitro required the presence of L2 and was susceptible to the same biochemical inhibitors, implying the cell-free assembled particles use the infectious pathway previously described for HPV16 produced in cell culture. Using biochemical and electron microscopy analyses, we observed that, in the presence of nuclear extract, intact VLPs partially disassemble, providing a mechanistic explanation to how the exogenous plasmid was packaged by these particles. Further, we provide evidence that capsids containing an <8-kb pseudogenome are resistant to the disassembly/reassembly reaction. Our results suggest a novel size discrimination mechanism for papillomavirus genome packaging in which particles undergo iterative rounds of disassembly/reassembly, seemingly sampling DNA until a suitably sized DNA is encountered, resulting in the formation of a stable virion structure.

IMPORTANCE Little is known about papillomavirus assembly biology due to the difficulties in propagating virus in vitro. The cell-free assembly method established in this paper reveals a new mechanism for viral genome packaging and will provide a tractable system for further dissecting papillomavirus assembly. The knowledge gained will increase our understanding of virus-host interactions, help to identify new targets for antiviral therapy, and allow for the development of new gene delivery systems based on _in vitro_-generated papillomavirus vectors.

Cell-free synthesis of encephalomyocarditis virus

Yuri V Svitkin 1, Nahum Sonenberg

Affiliations expand

Free PMC article

Abstract

We developed a system for complete replication of encephalomyocarditis virus (EMCV) in a test tube by using an in vitro translation extract from Krebs-2 cells. Efficient virus synthesis occurred in a narrow range of Mg(2+) and EMCV RNA concentrations. Excess input RNA impaired RNA replication and virus production but not translation. This suggests the existence of a negative-feedback mechanism for regulation of RNA replication by the viral plus-strand RNA or proteins.

Expand all items >>

20210316

Contents
  1. 1. Working directories
    1. 1.1. Installation Log in X1
    2. 1.2. Migration of Hexo
    3. 1.3. Hexo installation in X1
    4. 1.4. Laptop selection
    5. 1.5. Flatpak pack for frequently used Windows app’s alternatives
    6. 1.6. Downloaded music
  2. 2. Check information of debian package

Working directories

Installation Log in X1

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# Obsidian.md installation
flatpak install flathub md.obsidian.Obsidian
#
sudo apt-get install typora
#
sudo apt install subversion # commands of SVN
#
# Google Chrome/谷歌访问助手_v2.3.0.crx installation
i='/media/ht/ht_5T/Stored_Softs/Cross_Platform_Softs/Google_Chrome/Extensions/Selected'; cd ${i}; pcmanfm -n ${i}
#
sudo apt install curl
#
i='https://dl.google.com/linux/direct/google-chrome-stable_current_amd64.deb'
# Download from Google Chrome/ Version, 89.0.4389.90-1
#
cd /media/ht/ht_5T/Stored_Softs/Cross_Platform_Softs/WPS/Installer
sudo gdebi wps-office_10.1.0.6758_amd64.deb # Thinkpad X1
#
cd /media/ht/ht_5T/Stored_Softs/Cross_Platform_Softs/MS_Project_Alternative
sudo apt install planner
#
cd /media/ht/ht_5T/Stored_Softs/Cross_Platform_Softs/MS_Project_Alternative/ProjectLibre
sudo gdebi projectlibre_1.9.3-1.deb
#
sudo apt install xdotool
##################################################
# Installation of copyq from My Linux
#
sudo add-apt-repository ppa:noobslab/indicators
sudo apt-get update
sudo apt-get install copyq
##################################################
# Installation of pdftk with local files from PDF_Learning
#
sudo gdebi /media/ht/ht_5T/Stored_Softs/Linux_Softs/Not_Classified/libgcj-common_6.4-3ubuntu1_all.deb
sudo gdebi /media/ht/ht_5T/Stored_Softs/Linux_Softs/Not_Classified/libgcj17_6.4.0-8ubuntu1_amd64.deb
sudo gdebi /media/ht/ht_5T/Stored_Softs/Linux_Softs/Not_Classified/pdftk_2.02-4build1_amd64.deb
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install.packages(c("ape", "assertthat", "backports", "BH", "cli", "colorspace", "crayon", "crosstalk", "digest", "dplyr", "DT", "evaluate", "fansi", "ggplot2", "glue", "gtable", "highr", "htmltools", "htmlwidgets", "httpuv", "jsonlite", "knitr", "labeling", "later", "lazyeval", "magrittr", "markdown", "mime", "pander", "pillar", "pkgconfig", "plyr", "promises", "purrr", "R6", "Rcpp", "readxl", "reshape2", "rlang", "rmarkdown", "rprojroot", "rvcheck", "scales", "shiny", "stringi", "stringr", "tibble", "tidyr", "tidyselect", "tidytree", "tinytex", "utf8", "withr", "xfun", "xtable", "yaml"))

Migration of Hexo

Hexo installation in X1

Laptop selection

Flatpak pack for frequently used Windows app’s alternatives

Downloaded music

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- Ed Sheeran, Kygo, Avicii, Martin Garrix, The Chainsmokers, Dua Lipa Styles - Feeling Me #3
https://youtu.be/aEKjvJWocRM

2020年10月26日发布

Ed Sheeran, Kygo, Avicii, Martin Garrix, The Chainsmokers, Dua Lipa Styles - Feeling Me #3
All songs in this spotify playlist: https://spoti.fi/2TJ4Dyj​
#queenclub​ #deephouse2020​ #bestofdeephouse2020​

◢Follow Queen Club:
★ Youtube: https://fanlink.to/YouTubeQueenClub​
★ Instargram: www.instagram.com/deep_house_club
★ Twitter: https://twitter.com/deephouse_club​
★ Facebook: https://bit.ly/3dzyPXD​

◢Tracklist: 
0. Dj Goja x Lunis - Crazy
https://deepuniverse.fanlink.to/Crazy​
1. It's Love
https://fanlink.to/ItsLoveSolven​
2. Losing Touch
https://fanlink.to/LosingTouch​
3. Heartbeat - Hazzmet
https://fanlink.to/HeartbeatHazzmet​
4. Jon Sine - Stay feat. Gavin Beach
https://spoti.fi/3gVQtWi​
5. Jon Sine - Dawning
https://spoti.fi/2QMQYHD​
6. Gabriel Wittner - Fantasy 
https://spoti.fi/2ZNhB4m​
7. Nora Van Elken - Needed You (feat. Trove)
https://fanlink.to/noraneededyou​
8. Nora Van Elken - All Night Long
https://fanlink.to/allnightlongnora​
9. Nico Filippe - Where
https://bit.ly/3hbUAPe​
10. Under Glass - Alone
https://spoti.fi/37vgdG1​
11. LYOD - On My Way (ft. Tom Auton) 
https://smarturl.it/lyod-on-my-way​
12. LYOD, The Bayton Fields - Do It For Love
https://spoti.fi/2F37PmY​
13. Miza & Peter - Us Feat. Chris Linton
https://spoti.fi/2Bow8u8​
14. Mourad Hany - Moving On
https://EpicTonesRecords.lnk.to/9FJ81ZqZ​
15. Pascal Junior - Feelings
https://EpicTonesRecords.lnk.to/l8zxF​
16. Pascal Junior - If I Wouldn't Know (Aldi Be Cool Remix)
https://EpicTonesRecords.lnk.to/btsuXi9W​
17. Alexander Hristov - The Most (Andrey Kravtsov Remix)
https://spoti.fi/3lWJAYD​
18. SVET - Somebody (Original Mix) 
https://spoti.fi/2MMwo87​
19. Pete Bellis & Tommy - I Was Loving You
https://fanlink.to/ddr21​
20. Marc Philippe - September Rain 
https://fanlink.to/ddr92​
21. GeoM - Ashes (Original Mix)
https://fanlink.to/ddra8​
22. GeoM - Back To You (Marc Philippe Remix)
https://fanlink.to/ddr86​
23. Pete Bellis & Tommy - Do You Wanna Know (Marc Philippe Remix)
https://fanlink.to/ddr47​
24. Pete Bellis & Tommy - Treat Me Right (Marc Philippe Remix)
https://fanlink.to/ddr32​
25. Marc Philippe - Deepest Blue
https://fanlink.to/dtr533​
26. GeoM - Ashes (Original Mix)
https://fanlink.to/ddra8​

🌿 Follow Deep Disco Records 
➤ Spotify → https://spoti.fi/2U7HoTN​
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Youtube: https://bit.ly/2Be7c8p​
Spotify: https://spoti.fi/2VmRH4J​


"🌿 Video by Charles Gambino
Instagram - @CharlesGambino 
Vimeo - https://bit.ly/2V5FwK7​
Website - www.charlesguinto.com"

"🌿 Video By: Gaëtan Piolot
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»Instagram: https://bit.ly/2RsLWR0​ "
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♦ If any producer, label, artist or photographer has an issue with any of the music or video uploads please contact us and we will remove your work immediately. Thank you!
♦ We strive to find the best and most enjoyable music for you guys! We hope to make your days more beautiful with the music we share ! Peace love and music.
I'm also a music producer, I'm working on this project !
🚫 If you have any problem with copyright issues, or question please do not report me, take your time to contact us via mail, and we will response within 48h - 
💌 channel@queenmusik.com 💌
----------------------------------------------------------------------
» Thank you for watching! Have a nice day!
» Do not forget LIKE-SHARE-COMMENT
For copyright issues, please contact us or leave us a personal message. We will remove your material immediately. PLEASE do not choose a Youtube strike.

- FEMALE VOCAL TRANCE 2021 [FULL ALBUM]
https://youtu.be/FWGyiSTQ0yo

2021年2月20日发布

For more Trance: https://RazNitzan.lnk.to/RNMSpotify​
Subscribe to our Youtube Channel: http://bit.ly/2jNse3f​
FEMALE VOCAL TRANCE 2021

00:00​ | 1. Aurosonic & Fenna Day - You'll Be Fine
https://RazNitzan.lnk.to/YoullBeFineYD​
04:07​ | 2. C-Systems & Hanna Finsen - Feeling Fine
https://RazNitzan.lnk.to/FeelingFineYD​
07:49​ | 3. Costa & Sarah Russell - I Remember
https://RazNitzan.lnk.to/IRememberYD​
11:20​ | 4. Zetandel & Tiff Lacey - Higher Ground (Costa & Aurosonic Remix)
https://RazNitzan.lnk.to/HigherGround...​
14:58​ | 5. Stine Grove & Raz Nitzan - Snow Angels
https://RazNitzan.lnk.to/SnowAngelsYD​
18:30​ | 6. 4 Strings & Susanne Teutenberg - Hard To Face The Truth
https://RazNitzan.lnk.to/BrandNewDayA...​
21:58​ | 7. Susana - Dark Side of The Moon (Ferry Tayle Remix)
https://RazNitzan.lnk.to/DarkSideOfTh...​
25:42​ | 8. Ana Criado & Beat Service - Whispers (Kaimo K Remix)
https://RazNitzan.lnk.to/WhispersKaim...​
29:30​ | 9. Steve Allen, Trance Classics & Meredith Bull - The Love In Your Lies
https://RazNitzan.lnk.to/TheLoveInYou...​
33:18​ | 10. Jericho Frequency & Maxine - Touched By Love
https://RazNitzan.lnk.to/TouchedByLoveYD​
36:39​ | 11. Tycoos & Jan Johnston - The Landslide
https://RazNitzan.lnk.to/TheLandslideYD​
39:40​ | 12. Michael Angelo - Forever In Your Arms
https://RazNitzan.lnk.to/ForeverInYou...​
43:05​ | 13. Steve Allen, Paul Skelton & Deirdre McLaughlin - Fairytales
https://RazNitzan.lnk.to/FairytalesYD​
46:36​ | 14. Suncatcher & Exolight - Water Colour Memories (Last Soldier Remix)
https://RazNitzan.lnk.to/WaterColourM...​
50:30​ | 15. 4 Strings, Trance Classics & Ellie Lawson - Safe From Harm (Omar Sherif Remix)
https://RazNitzan.lnk.to/BecomingAlbumYD​
53:45​ | 16. Michael Fearon feat. Victoria Leshchenko - Supernatural
https://RazNitzan.lnk.to/SupernaturalYD​
57:11​ | 17. Dmitry Strochenko & Maxine - Daylight Snow
https://RazNitzan.lnk.to/DaylightSnowYD​
1:00:37​ | 18. Costa & Tricia McTeague - Call I'll Be There
https://RazNitzan.lnk.to/CallIllBeThe...​
1:04:04​ | 19. Raz Nitzan & Fenna Day - Room For Doubt (Stoneface & Terminal Remix)
https://RazNitzan.lnk.to/RoomForDoubt...​
1:07:35​ | 20. Sarah Russell & Philippe El Sisi - You Are (Steve Dekay Remix)
https://RazNitzan.lnk.to/YouAreSteveD...​
1:11:03​ | 21. Michael Angelo & Solo feat. Chloe Langley - Every Time We Said Goodbye 2021
https://RazNitzan.lnk.to/EveryTimeWeS...​
1:14:21​ | 22. Mhammed El Alami & Cathy Burton - Never Fade Away
https://RazNitzan.lnk.to/NeverFadeAwayYD​
1:17:36​ | 23. Koishii & Hush feat. Gillian Gilbert - Lifetime (Passenger 75 Remix)
https://RazNitzan.lnk.to/LifetimeRemi...​
1:21:02​ | 24. Bobina & Elles de Graaf - Lighthouse (Sean Tyas Edit)
https://RazNitzan.lnk.to/LighthouseRmxYD​
1:24:27​ | 25. Andy Prinz & Naama Hillman - Quiet of Mind (Aly & Fila Remix)
https://RazNitzan.lnk.to/QuietOfMindYD​
P&C 2021 RAZNITZANMUSIC / RNM BUNDLES

PARTNER CHANNELS:
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Copyright © 2021 by RazNitzanMusic (RNM)
#VocalTrance2021​ #Trance​ #UpliftingTrance​

- Dj Dark - Summer Chill (August 2020) [Deep, Vocal, Chill Mix]
https://youtu.be/widtdkzOv1E
2020年8月29日发布

▶  Subscribe & turn ON the 🔔 : http://short.djdark.ro/subscribe​
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▶  Download + Tracklist: https://djdark.ro/?p=14251​

✈ Booking: +4 0723 548 116 / booking@bestshow.ro
☑️ Listen on SoundCloud: http://short.djdark.ro/summerchill​
☑️ DOWNLOAD [Link 2]: http://short.djdark.ro/summerchillyt​

✔️ Follow Me:
↪︎ http://short.djdark.ro/socials​

↪︎ Tracklist:
00:00​ Alvin Whitte, Ovrtherainbovv - Be Happy
03:42​ Jubel feat.NEIMY - Dancing In The Moonlight
06:14​ A-Mase & Sharliz, Ladynsax - Crush (Original Mix)
11:01​ Lady Gaga, Ariana Grande - Rain On Me (Dj Dark & Mentol Rework)
14:25​ Lewis Capaldi - Before You Go (Shine Remix)
17:49​ David Guetta feat.Sia - Titanium (A-Mase's Ibiza Sunset Remix)
24:10​ Formal Chicken - Push The Feeling On (Extended)
26:48​ Fugees, Dave Delly - Killin' Me Softly (Dave Delly Remix)
30:09​ Jason Derulo - Savage Love (Dj Dark & Mentol x D.E.P. Remix)
33:34​ Jay Ko & Narciss x David Deejay - Sexy Thing (Extended Remix)
36:39​ Melih Aydogan - Ayo Technology (The Distance & Igi Remix)
41:05​ MoonSound x Rachael Starr - Till There Was You (Extended)
44:12​ Nora En Pure - Delta (Extended Mix)
48:10​ The Suncatchers - On My Own (Alex Vnuk Remix)
51:00​ Regard, Raye - Secrets (Consoul Trainin Remix)
54:06​ Crazibiza - Cali Soul (Extended)
57:14​ Jon Costa, David Novacek - Couldn't Love You More (Mr. Moudz Remix)
01:00:13​ Yann Muller - Paroles (Original Mix)

_________________________________________________________________
🚫 If you have any problem with copyright issues, or question please do not report me, take your time to contact us via mail, and i will respond within 48h -
💌 contact@djdark.ro 💌

#djdark​ #summerchill​ #deephouse​

Check information of debian package

1
dpkg --info xxx.deb

Expand all items >>

20210314

Contents
  1. 1. Working directories
    1. 1.1. 符号大全
    2. 1.2. 12 Best Microsoft Flow Alternatives - Reviews, Features, Pros & Cons - Alternative
    3. 1.3. Offline Wikipedia
      1. 1.3.1. Wikipedia:Database download
      2. 1.3.2. Kiwix
  2. 2. Install with flatpak
    1. 2.0.1. WebArchives
  • 3. 糖尿病

    • Working directories

    1
    2
    /home/ht/AppImage/Obsidian-0.11.5.AppImage
    ${HOME}/AppImage/Obsidian-*.AppImage

    符号大全

    12 Best Microsoft Flow Alternatives - Reviews, Features, Pros & Cons - Alternative

    Offline Wikipedia

    I tried lots in Linux environment.

    Wikipedia:Database download

    Kiwix

    ZIM format document needed.

    Install with flatpak

    1
    2
    3
    flatpak install flathub org.kiwix.desktop
    flatpak run org.kiwix.desktop
    flatpak run org.kiwix.desktop

    WebArchives

    Installation from GitHub - birros/web-archives: A web archives reader

    1
    2
    3
    4
    flatpak remote-add flathub https://flathub.org/repo/flathub.flatpakrepo
    flatpak install flathub com.github.birros.WebArchives
    #
    flatpak run com.github.birros.WebArchives

    糖尿病

    To be organized

    1
    2
    3
    i='/media/ht/ht_5T/Work/References/Diabetes'
    cd ${i}
    pcmanfm -n ${i}
    • 1.list, download list from 中华医学会糖尿病学学分会, colleciton of guidelines
    • 6360650853146500005447651.pdf
    • 6360650858691812504989121.pdf
    • 6360650875834000003259241.pdf
    • 6360650885093375008213363.pdf
    • 6360650891598062506418654.pdf
    • 6360650900034000003924937.pdf
    • 国家基层糖尿病防治管理指南-2018.pdf
    • 老年人糖尿病前期干预指南-2018.pdf
    • 糖尿病-维基百科.pdf
    • 糖尿病_Learning.docx
    • 糖尿病.pdf
    • 中国2型糖尿病防治指南-2010.pdf
    • 中国 2 型糖尿病防治指南-2017.pdf
    • 中国2型糖尿病防治指南_基层版-2012.pdf
    • 中国糖尿病防治指南_科普版-2009.pdf
    • 中国I型糖尿病诊治指南-2011.pdf
    • CHN_D1_Diabetes-prevention-guidelines.pdf
    • Diabetes.pdf
    • Diabetes-Wikipedia.pdf

    Expand all items >>

    20210312

    Contents
    1. 1. Working directories
      1. 1.1. Skill levels and gains in university STEM education in China, India, Russia and the United States | Nature Human Behaviour

    Working directories

    Skill levels and gains in university STEM education in China, India, Russia and the United States | Nature Human Behaviour

    Universities contribute to economic growth and national competitiveness by equipping students with higher-order thinking and academic skills. Despite large investments in university science, technology, engineering and mathematics (STEM) education, little is known about how the skills of STEM undergraduates compare across countries and by institutional selectivity. Here, we provide direct evidence on these issues by collecting and analysing longitudinal data on tens of thousands of computer science and electrical engineering students in China, India, Russia and the United States. We find stark differences in skill levels and gains among countries and by institutional selectivity. Compared with the United States, students in China, India and Russia do not gain critical thinking skills over four years. Furthermore, while students in India and Russia gain academic skills during the first two years, students in China do not. These gaps in skill levels and gains provide insights into the global competitiveness of STEM university students across nations and institutional types.

    Expand all items >>

    20210311

    Contents
    1. 1. NSFC Application
    2. 2. To be organized
    3. 3. Linux 搜狗输入法 隐藏状态栏_写了 = 会了.gif-CSDN博客

    NSFC Application

    1
    2
    3
    4
    5
    6
    #
    i='/media/ht/ht_5T/Work/Funding/2021_国家自然科学基金'; pcmanfm -n ${i}; cd ${i} # NSFC
    #
    i='/media/ht/ht_5T/Work/Funding/2021_国家自然科学基金/Draft/1.面上项目-填报说明与撰写提纲-20201228版_Filled.doc'; setsid wps ${i} # Guideline
    #
    i='/media/ht/ht_5T/Work/Funding/2017_重大专项/Management/Administration/09_02_63_HEV/Ref/Taxonomy'; pcmanfm -n ${i}; cd ${i} # Ref of HEV

    To be organized

    [[20210329|## To be organized]]

    1
    i='/media/ht/ht_5T/Work/References/Computer_Tips/Google_Tips/1';cd ${i}; pcmanfm -n ${i}

    Linux 搜狗输入法 隐藏状态栏_写了 = 会了.gif-CSDN博客

    Expand all items >>

    20210310

    Contents
    1. 1. Working directories
      1. 1.1. Backup
      2. 1.2. Viral load of EBV activation in B95-8
        1. 1.2.1. Original data pack from LI Ying
        2. 1.2.2. Data treatment
      3. 1.3. Movie from RS and Mate
    2. 2. 移动硬盘
      1. 2.1. WD My Wireless Pro移动硬盘
      2. 2.2. Samsung 移动硬盘
    3. 3. QQ信息

    Working directories

    Backup

    1
    2
    3
    Source='/media/ht/ht_5T/Work/'
    Destination='/media/ht/ht_Mobile/Work/'
    rsync -auP --update ${Source} ${Destination}

    Viral load of EBV activation in B95-8

    1
    2
    i='/media/ht/ht_5T/Work/Projects/NFE/B95-8/qPCR'; cd ${i}; pcmanfm -n ${i}
    #

    Original data pack from LI Ying

    • user-20210223142143.pdf
    • user-20210223142143.tlpd
    • user-20210223142143 (阶段3 - 步骤3) - Quantitation Amplification Results.xlsx
    • user-20210223142143 (阶段3 - 步骤3) - Quantitation Ct Results.xlsx
    • user-20210223142143 (阶段3 - 步骤3) - Quantitation Raw Curve Results.xlsx

    Data treatment

    • 20210223_RT-PCR.7z, Original pack from LI Ying
    • user-20210223142143_3-Quantitation_Amplification_Results.xlsx
    • user-20210223142143_3-Quantitation_Ct_Results.xlsx, treated
    • user-20210223142143_3-Quantitation_Raw_Curve_Results.xlsx
    • user-20210223142143.pdf
    • user-20210223142143.tlpd
    • 20210223_3-Amplification_Results.xlsx
    • 20210223_3-Ct_Results.xlsx
    • 20210223_3-Raw_Curve_Results.xlsx
    • 20210223.pdf
    • 20210223_RT-PCR.7z
    • 20210223.tlpd
    1
    2
    3
    setsid et /media/ht/ht_5T/Work/Projects/NFE/B95-8/qPCR/20210223/20210223_3-Ct_Results.xlsx
    #
    i='/media/ht/ht_5T/Work/Projects/NFE/B95-8/qPCR/20210223/20210223_3-Ct_Results.xlsx'; setsid et ${i}

    Movie from RS and Mate

    To be organized

    The above note will be deleted after HD organization

    1
    i='/media/ht/ht_5T_9/YouTubes/IT/Not_Classified/20210310'; cd ${i}; pcmanfm -n ${i}
    • How_To_Reset_Air_1_Standard_Edition_Happy_Plugs.mp4
    • y2mate.com-Yaizu_City_Japan_8K_焼津市_1080p.mp4
    • y2mate.com-破解美式英语三大关键因素中式英语真的菜吗未必哟5_March_2021_480p.mp4
    • y2mate.com-硬盘加密保护隐私文件加密veracrypt的完整使用指南_360p.mp4
    • 党史杂谈_437_—毛在进京之后再没有回访延安的谜团_老邓的“严打”与老周的“一打三反”.mp4
    • 湖北省老人津貼去年第一季度少了15萬人_這個數字確實嗎_財新網稱武漢死亡率較預期增加56%_這篇報導為何被刪除_死亡數字其實沒有什麼見不得人的_但為何就不能公諸於世呢.mp4
    • 让子弹飞全网最详细拉片三小时长文解析.mp4
    • 真正的自动剪辑神器_完全免费开源_无任何功能限制_Youtuber快速制作视频必备工具!_零度解说.mp4

    移动硬盘

    Keywords

    • WD My Wireless Pro移动硬盘

    WD My Wireless Pro移动硬盘

    See 好物推荐 — — WD My Wireless Pro移动硬盘. 熟悉我的人都知道我对于准Geek装备，绝对属于败家的。在这一条看似与单反相近的“… | by Josh Cheng | Medium

    Samsung 移动硬盘

    QQ信息

    • 垂直胶上样时，相邻两泳道的上样量（微升数）必须保持一致。水平较，在进行精细实验实验时也要应用上述上样原则。上样量少的，使用1.25XLoading Buffer补足。

    Expand all items >>

    20210309

    Contents
    1. 1. Working directories
      1. 1.1. HFMD, STGCN Model Paper

    Working directories

    1
    2
    3
    i='/media/ht/ht_5T/Work/Projects/Exosome/Publication/HSV_cn/20210309'; cd ${i}; pcmanfm -n ${i} # moved files from RS QQ
    #
    i='/media/ht/ht_5T/Family/XYZ/20210221/'; cd ${i}; pcmanfm -n ${i}

    HFMD, STGCN Model Paper

    1
    2
    i='/media/ht/ht_5T/Work/Funding/2017_重大专项/Management/Administration/05_00_HFMD/Publication'; cd ${i}; pcmanfm -n ${i} # HFMD Publication
    #
    • STGCN_Model_20210308.zip, received from Ji Tianjiao
    • STGCN_Model_20210308.docx
    • STGCN_Model-Supp_20210308.docx
    1
    2
    3
    i='/media/ht/ht_5T/Work/Funding/2017_重大专项/Management/Administration/05_00_HFMD/Publication/STGCN_Model_20210308.docx'; setsid wps ${i}
    #
    i='/media/ht/ht_5T/Work/Funding/2017_重大专项/Management/Administration/05_00_HFMD/Publication/STGCN_Model-Supp_20210308.docx'; setsid wps ${i}

    Expand all items >>

    20210308

    Contents
    1. 1. Working directories
      1. 1.1. To be organized
      2. 1.2. 基因室进展汇报
      3. 1.3. Summary of EBV activation conditions
      4. 1.4. Viral load of EBV in B95-8

    Working directories

    To be organized

    基因室进展汇报

    1
    2
    3
    4
    5
    i='/media/ht/ht_5T/Work/肿瘤室工作/基因室文件/20210308'
    cd ${i}
    #
    i='/media/ht/ht_5T/Work/肿瘤室工作/基因室文件/20210308/年报excel_肿瘤室.7z'
    ls ${i} # received from LI Ying's QQ
    • 标志性成果模板（限5项）.xlsx
    • 成果转化模板.xlsx
    • 奖励情况模板_肿瘤室.xlsx
    • 论文清单模板-1_肿瘤室.xlsx
    • 年报excel_肿瘤室.7z
    • 年度工作运行模板_肿瘤室.docx
    • 人才项目荣誉情况模板.xlsx
    • 申请专利清单模板_肿瘤室.xlsx
    • 授权专利清单模板.xlsx
    • 项目经费情况模板_肿瘤室.xlsx
    • 学术交流模板(参加).xlsx
    • 学术交流模板(主办).xlsx

    Summary of EBV activation conditions

    1
    2
    3
    4
    5
    6
    7
    i='/media/ht/ht_5T/Work/Projects/NFE/B95-8/Summary'; cd ${i}
    #
    ln -s B95-8_Nrf2_Project_20210303.pptx B95-8_Nrf2_Project.pptx
    #############################################
    # Summary of B95-8_Nrf2 Project
    #############################################
    i='/media/ht/ht_5T/Work/Projects/NFE/B95-8/Summary/B95-8_Nrf2_Project.pptx'; setsid wpp ${i}

    Viral load of EBV in B95-8

    Expand all items >>

    {% if theme.mermaid.enable %} {% endif %}

    tag:

      缺失模块。
      1、请确保node版本大于6.2
      2、在博客根目录（注意不是yilia根目录）执行以下命令：
      npm i hexo-generator-json-content --save

      3、在根目录_config.yml里添加配置： 

        jsonContent:
    meta: false
    pages: false
    posts:
      title: true
      date: true
      path: true
      text: false
      raw: false
      content: false
      slug: false
      updated: false
      comments: false
      link: false
      permalink: false
      excerpt: false
      categories: false
      tags: true

    Step by step.