20220125

Contents

1. 1. Working directories
2. 2. RPA probe
   1. 2.1. Order forms
3. 3. 奇葩的批量转换
4. 4. 引物整理
5. 5. miRDeep2, miR Prediction

Working directories

RPA probe

• p96 in summarized PDF document

# p1489 B2

Order forms

i='/media/ht/ht_5T/Work/References/Manufacturer_Manual/TwistDx/Handbooks/Oligo_Order'; \
cd ${i}

---

├── Basic_oligonucleotide_forms
│   ├── 1_btioligoorder8f2d67a1f42264a8875eff0000c536f5.xls
│   ├── 2_eurogentec-oligo-batch-order-v8-7-twistamp.xlsx
│   └── Basic_oligonucleotide_forms.7z
├── exo_oligonucleotide_forms
│   ├── 1_btioligoorder8f2d67a1f42264a8875eff0000c536f5-1.xls
│   ├── 2_eurogentec-oligo-batch-order-v8-7-twistamp-2.xlsx
│   ├── 3_twistamp_exo_order_form_biosearch.xls
│   ├── 4_twistamp_exo_order_form_generic.xls
│   └── exo_oligonucleotide_forms.7z
├── fpg_oligonucleotide_forms
│   ├── 1_btioligoorder8f2d67a1f42264a8875eff0000c536f5.xls
│   ├── 2_eurogentec-oligo-batch-order-v8-7-twistamp.xlsx
│   ├── 3_twistamp_fpg_order_form_biosearch.xls
│   ├── 4_twistamp_fpg_order_form_generic.xls
│   └── fpg_oligonucleotide_forms.7z
└── nfo_oligonucleotide_forms
    ├── 1_btioligoorder8f2d67a1f42264a8875eff0000c536f5-1.xls
    ├── 2_eurogentec-oligo-batch-order-v8-7-twistamp.xlsx
    ├── 3_twistamp_nfo_order_form_biosearch.xls
    ├── 4_twistamp_nfo_order_form_generic.xls
    └── nfo_oligonucleotide_forms.7z

4 directories, 18 files

├── Basic_oligonucleotide_forms
│   ├── 1_btioligoorder8f2d67a1f42264a8875eff0000c536f5.xls
│   ├── 2_eurogentec-oligo-batch-order-v8-7-twistamp.xlsx
│   └── Basic_oligonucleotide_forms.7z
├── exo_oligonucleotide_forms
│   ├── 1_btioligoorder8f2d67a1f42264a8875eff0000c536f5-1.xls
│   ├── 2_eurogentec-oligo-batch-order-v8-7-twistamp-2.xlsx
│   ├── 3_twistamp_exo_order_form_biosearch.xls
│   ├── 4_twistamp_exo_order_form_generic.xls
│   └── exo_oligonucleotide_forms.7z
├── fpg_oligonucleotide_forms
│   ├── 1_btioligoorder8f2d67a1f42264a8875eff0000c536f5.xls
│   ├── 2_eurogentec-oligo-batch-order-v8-7-twistamp.xlsx
│   ├── 3_twistamp_fpg_order_form_biosearch.xls
│   ├── 4_twistamp_fpg_order_form_generic.xls
│   └── fpg_oligonucleotide_forms.7z
└── nfo_oligonucleotide_forms
├── 1_btioligoorder8f2d67a1f42264a8875eff0000c536f5-1.xls
├── 2_eurogentec-oligo-batch-order-v8-7-twistamp.xlsx
├── 3_twistamp_nfo_order_form_biosearch.xls
├── 4_twistamp_nfo_order_form_generic.xls
└── nfo_oligonucleotide_forms.7z

奇葩的批量转换

• command line - Converting multiple image files from JPEG to PDF format - Unix & Linux Stack Exchange
• command line - Convert a directory of JPEG files to a single PDF document - Ask Ubuntu

以上两个链接中，真有神奇的命令。

convert *.jpg -auto-orient pictures.pdf
# 
convert *.jpg +adjoin page-%d.pdf

下面的更牛！居然可以OCR。

1. Make PDF

   img2pdf *.jp* --output combined.pdf

2. (optional) OCR the output PDF

   ocrmypdf combined.pdf combined_ocr.pdf

又知道一个新的软件

pdfjam --a4paper *.jpg

下面的Shell命令组合，让人神往

parallel convert '{} {.}.pdf' ::: *.jpg

A tiny script would do the trick. (tested with ksh88 on Solaris 10)

script.ksh

#!/bin/ksh

[[ $# != 1 ]] && exit 255 # test for nr of args

pdfname=$(sed -e 's/\(.*\)\.jpg/\1\.pdf/' <(echo $"1")) #replace *.jpg with *.pdf
convert "$1" $pdfname

Then you can run find to execute the script:

find dir -name image\*.jpg -exec /bin/ksh script.ksh {} \;

引物整理

cd /media/ht/ht_5T/Work/肿瘤室工作/日常管理/引物管理

miRDeep2, miR Prediction

cd /media/ht/ht_5T/Database/miR_Base/miR_Prediction/miRDeep2/Installation

赏

谢谢你请我吃糖果