- 1. Working directories
- 2. SNV and InDel Discovery in Chromatogram traces obtained from Sanger sequencing of PCR products.
- 3. Transpose a table
- 4. Deepin
- 5. Login error
- 6. 佛经
- 7. Bioinfo softwares
Working directories
SNV and InDel Discovery in Chromatogram traces obtained from Sanger sequencing of PCR products.
Transpose a table
- shell transpose table - Google Search
- parsing - An efficient way to transpose a file in Bash - Stack Overflow
- parsing - An efficient way to transpose a file in Bash - Stack Overflow
- awk - list to tables
- sed - How to transpose a list to a table in bash - Stack Overflow
Deepin
为了QQ实在不胜其烦。尤其是Deepin的那些东西。只好删除,使用原生的Linux QQ。
sudo apt purge *deepin-wine*
From ubuntu安装deepin-wine,如何删除?- Community - Deepin Technology.
Login error
1 | /etc/profile.d/01-locale-fix.sh |
佛经
1 | git clone https://github.com/gwsice/buddhism |
Bioinfo softwares
- SnapGene Viewer | Free software for plasmid mapping, primer design, and restriction site analysis
- Viewing Chromatograms < DNA Sequencing
- Vector NTI Alternatives and Similar Software | AlternativeTo
Linux
3
DNADynamo
Is this a good alternative?
Yes
No
Paid • Proprietary
Mac
Windows
Linux
-
DNADynamo - for Windows, OSX and Linux. Download it now for abi/scf trace alignments, sub cloning and plasmid management, blast/sequence retrieval, sequence annotation and structure viewing - all in one integrated and easy to use bioinformatics program.
2
Rasmol
Is this a good alternative?
Yes
No
Free • Open Source
Mac
Windows
Linux
-
RasMol is a computer program written for molecular graphics visualization intended and used primarily for the depiction and exploration of biological macromolecule structures, such as those found in the Protein Data Bank.
4
SnapGene Viewer
Is this a good alternative?
Yes
No
Free Personal • Proprietary
Mac
Windows
Linux
31 alternatives to SnapGene Viewer
SnapGene Viewer is revolutionary software that allows molecular biologists to create, browse, and share richly annotated DNA sequence files up to 1 Gb in length.
SnapGene Viewer vs Vector NTI opinions
pros, cons and recent comments
Gu
The free version does not offer any of the features one would find in vectorNTI
Negative comment • over 2 years ago
1
1
Clustal X
Is this a good alternative?
Yes
No
Free • Proprietary
Mac
Windows
Linux
-
Multiple sequence alignment software.
1
PDRAW32
Is this a good alternative?
Yes
No
Freemium • Proprietary
Mac
Windows
Linux
-
Scientific software for the molecular biologist. Performs virtual DNA cloning, analyses DNA for restriction enzymes, homing enzymes and primer sites, dam and dcm methylation sites, finds and translates open reading frames. Calculates annealing temperatures for PCR.
1
CLC Genomics Workbench
Is this a good alternative?
Yes
No
Paid • Proprietary
Mac
Windows
Linux
24 alternatives to CLC Genomics Workbench
Complete bioinformatics toolkit for a wide range of genomics analysis needs, including transcriptomics, variant calling, epigenomics, metagenomics, comparative genomics and many more.
1
Ridom TraceEdi
Is this a good alternative?
Yes
No
Free • Proprietary
Windows
Linux
9 alternatives to Ridom TraceEdi
Ridom TraceEdit is a cross-platform graphical DNA trace viewer and editor. TraceEdit displays the chromatogram files from Applied Biosystems automated sequencers and files in the Staden SCF format.
1
UGENE
Is this a good alternative?
Yes
No
Free • Open Source
Mac
Windows
Linux
UGENE is free open-source cross-platform bioinformatics software.
1
Geneious
Is this a good alternative?
Yes
No
Paid • Proprietary
Mac
Windows
Linux
Geneious combines all the major DNA and protein sequence analysis tools into one revolutionary software solution!
2
Serial Cloner
Is this a good alternative?
Yes
No
Free • Proprietary
Mac
Windows
Linux
Serial Cloner is a Molecular Biology software. It provides tools with an intuitive interface that assists you in DNA cloning, sequence analysis and visualization.
Discontinued
Last version, 2.6.1, was released on April 3rd, 2013.
Windows
3
DNADynamo
Is this a good alternative?
Yes
No
Paid • Proprietary
Mac
Windows
Linux
-
DNADynamo - for Windows, OSX and Linux. Download it now for abi/scf trace alignments, sub cloning and plasmid management, blast/sequence retrieval, sequence annotation and structure viewing - all in one integrated and easy to use bioinformatics program.
2
Rasmol
Is this a good alternative?
Yes
No
Free • Open Source
Mac
Windows
Linux
-
RasMol is a computer program written for molecular graphics visualization intended and used primarily for the depiction and exploration of biological macromolecule structures, such as those found in the Protein Data Bank.
2
BioEdit
Is this a good alternative?
Yes
No
Free • Proprietary
Windows
-
BioEdit is a biological sequence alignment editor written for Windows 95/98/NT/2000/XP/7. An intuitive multiple document interface with convenient features makes alignment and manipulation of sequences relatively easy on your desktop computer.
Discontinued
The program is no longer maintained. Last version, 7.2.5, released in December 2013, can be still downloaded from the official website.
4
SnapGene Viewer
Is this a good alternative?
Yes
No
Free Personal • Proprietary
Mac
Windows
Linux
31 alternatives to SnapGene Viewer
SnapGene Viewer is revolutionary software that allows molecular biologists to create, browse, and share richly annotated DNA sequence files up to 1 Gb in length.
SnapGene Viewer vs Vector NTI opinions
pros, cons and recent comments
Gu
The free version does not offer any of the features one would find in vectorNTI
Negative comment • over 2 years ago
1
0
SimVector
Is this a good alternative?
Yes
No
Paid • Proprietary
Mac
Windows
-
Use SimVector to draw plasmid maps, perform restriction analysis and mapping. The plasmid drawing software also simulates cloning experiments such as gateway cloning, ta cloning and restriction cloning
1
GeneDoc
Is this a good alternative?
Yes
No
Free • Proprietary
Windows
-
A full featured multiple sequence alignment editor, analyser and shading utility for Windows. • Data analysis and visualization • Score assisted manual alignment • Paginated printouts • Windows based • Highly configurable • Exported figures • Phylognetic tree support
1
Clustal X
Is this a good alternative?
Yes
No
Free • Proprietary
Mac
Windows
Linux
-
Multiple sequence alignment software.
1
PDRAW32
Is this a good alternative?
Yes
No
Freemium • Proprietary
Mac
Windows
Linux
Scientific software for the molecular biologist. Performs virtual DNA cloning, analyses DNA for restriction enzymes, homing enzymes and primer sites, dam and dcm methylation sites, finds and translates open reading frames. Calculates annealing temperatures for PCR.
1
CLC Genomics Workbench
Is this a good alternative?
Yes
No
Paid • Proprietary
Mac
Windows
Linux
Complete bioinformatics toolkit for a wide range of genomics analysis needs, including transcriptomics, variant calling, epigenomics, metagenomics, comparative genomics and many more.
1
Ridom TraceEdi
Is this a good alternative?
Yes
No
Free • Proprietary
Windows
Linux
Ridom TraceEdit is a cross-platform graphical DNA trace viewer and editor. TraceEdit displays the chromatogram files from Applied Biosystems automated sequencers and files in the Staden SCF format.
Aricles related to cloning and RE
- Primer Design for Restriction Enzyme Cloning (E6901) | NEB
- Primer Design for Restriction Enzyme Cloning (E6901)
- Primer Design for Restriction Enzyme Cloning (E6901)
- Primer Design for Restriction Enzyme Cloning (E6901)
- Restriction Site Extension PCR: A Novel Method for High-Throughput Characterization of Tagged DNA Fragments and Genome Walking
- A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends | BMC Biotechnology | Full Text
- Rapid Restriction Enzyme-Free Cloning of PCR Products: A High-Throughput Method Applicable for Library Construction
- Restriction Enzyme Resource Guide
- Recognition of Flanking DNA Sequences by EcoRV Endonuclease Involves Alternative Patterns of Water-mediated Contacts*
- Restriction enzymes & DNA ligase (article) | Khan Academy
Codon optimization
- codon optimization and expression level - Google Search
- Codon Optimization in the Production of Recombinant Biotherapeutics: Potential Risks and Considerations | SpringerLink
- Predicting Gene Expression Level from Codon Usage Bias | Molecular Biology and Evolution | Oxford Academic
- Strategies of codon optimization for high-level heterologous protein expression in microbial expression systems - ScienceDirect
- Codon usage is an important determinant of gene expression levels largely through its effects on transcription | PNAS
- Developing a codon optimization method for improved expression of recombinant proteins in actinobacteria | Scientific Reports
- Codon optimization with deep learning to enhance protein expression | Scientific Reports
- Codon Optimization Significantly Improves the Expression Level of α-Amylase Gene from Bacillus licheniformis in Pichia pastoris